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Tony's Intertwingled Memex • Reference Manual

Bioreactor Substrate Yield Log (Chemical & Biomedical Engineering)

Formulas & Definitions

A biochemical engineer is monitoring an automated pilot bioreactor for synthetic therapeutic protein production. The automated process control system logs discrete sensor telemetry for microbial biomass concentration X(t)X(t)X(t) (in g/L\text{g/L}g/L) and residual substrate glucose concentration S(t)S(t)S(t) (in g/L\text{g/L}g/L), along with their respective rates of change:

Time t (hr)t\text{ (hr)}t (hr) X(t) (g/L)X(t)\text{ (g/L)}X(t) (g/L) S(t) (g/L)S(t)\text{ (g/L)}S(t) (g/L) X′(t) (g/(L⋅hr))X'(t)\text{ (g/(L}\cdot\text{hr))}X′(t) (g/(L⋅hr)) S′(t) (g/(L⋅hr))S'(t)\text{ (g/(L}\cdot\text{hr))}S′(t) (g/(L⋅hr))
2 1.51.51.5 18.018.018.0 0.40.40.4 −2.1-2.1−2.1
4 2.82.82.8 12.512.512.5 0.90.90.9 −3.5-3.5−3.5
6 5.25.25.2 5.05.05.0 1.21.21.2 −4.0-4.0−4.0
8 6.86.86.8 1.21.21.2 0.30.30.3 −1.8-1.8−1.8
  1. The instantaneous biomass-to-substrate conversion ratio is defined by Y(t)=X(t)S(t)Y(t) = \frac{X(t)}{S(t)}Y(t)=S(t)X(t)​. Write down the formal structural Quotient Rule formula for dYdt\frac{dY}{dt}dtdY​.
  2. Evaluate the instantaneous rate of change of the conversion ratio at peak exponential growth (t=4 hrt = 4\text{ hr}t=4 hr).
  3. The total biochemical activity index is given by A(t)=t⋅X(t)A(t) = t \cdot X(t)A(t)=t⋅X(t). Use the Product Rule to calculate A′(6)A'(6)A′(6).